u937 atcc crl 1593 2 human (ATCC)

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ATCC u937 atcc crl 1593 2 human
U937 Atcc Crl 1593 2 Human, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 6862 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/atcc+crl+1593+2/U-937/pm41923618-208-129-130
Average 99 stars, based on 6862 article reviews

u937 atcc crl 1593 2 human - by Bioz Stars, 2026-09

99/100 stars

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Modification:

Article Title: Gold Nanoparticles to Ameliorate Obesity Related Glucose Intolerance
Article Snippet: .. Table 3.1 Details of materials used for macrophage and adipocyte cultures Materials Catalogue number/supplier Mammaiian cell lines: Murine RAW264.7 macrophage (RM®), adherent Human U937 monocyte (UM®), non-adherent Murine 3T3-L1 fibroblast, adherent Culture media: Dulbecco's Modified Eagle's Medium (DMEM) Roswell Park Memorial Institute (RPMI) 1640 Media supplements/chemicals: Heat-inactivated Fetal Bovine Serum (FBS) Penicillin and Streptomycin (P/S, antibiotics) Phorbol 12-Myristate 13-Acetate (PMA) Lipopolysaccharide (LPS) New Calf Serum (NCS), New Zealand Insulin Dexamethasone 3-Isobutyl-1-Methly-Xanthine Biotin Buffer solutions: Dulbecco's Phosphate Buffered Saline (DPBS) Trypsin-Ethylene Diamine Tetraacetic Acid solution (Trypsin-EDTA) N-2-Hydroxyethylpiperazine-N-2-Ethane Sulfonic acid buffer (HEPES) CL-173, ATCC® CRL-1593.2, ATCC® TIB-71, ATCC® D5796, Sigma Aldrich 11875-093, Life Technologies 10082-147, Life Technologies 5140-122, Life Technologies P8139, Sigma Aldrich L7770, Sigma Aldrich 16010-159, Life Technologies 10516, Sigma Aldrich D4902, Sigma Aldrich 15879, Sigma Aldrich B4639, Sigma Aldrich 14190-144, Life Technologies 15400-054, Life Technologies 15630-080, Life Technologies ATCC®, Manassas, VA, USA; Sigma Aldrich, St. Louis, MO, USA; Life Technologies, Carlsbad, CA, USA Page 75 ..

Saline:

Concentration Assay:

Article Title: Methods and compositions for T-cell coculture potency assays and use with cell therapy products
Article Snippet: .. Step Parameter Description Stimulation Assay format 4-point dose U937 vs. IFN-γ concentration curve Assay throughput 48-well plate Activation cell line U937, ATCC CRL-1593.2 (lot no. 70036658) Number of TILs per well 1.5 × 106 Number of U937 cells per well 4.0 × 105, 2.0 × 105, 1.0 × 105, 0.5 × 105 Co-culture media AIM-V (serum-free medium) containing IL-2 (300 IU/mL) Volume of media 1000 μL Number of replicate wells per 3 (all three wells are sourced from the same thawed vial of test article; an sample/per plate/per run average of 3 wells is used to determine IFNγ concentration). .. Period of stimulation 24 ± 0.5 hours at 37° C. in an incubator with 5% CO2 Positive control Qualified TIL line Negative control Anti-HLA I&II antibodies Analysis Assay throughput 72-well plate Instrument ELLA automated ELISA System Software Simple Plex Runner and Simple Plex Explorer ELLA parameters Simple Plex 72-well IFN-γ 3rd generation Version 5 cartridge (cat. no. SPCKB-CS-002697), ProteinSimple, paired capture and detection antibodies conjugated to three (3) glass microtubes in series for triplicate repeated readout within each well.

Article Title: Methods and compositions for T-cell coculture potency assays and use with cell therapy products
Article Snippet: .. Step Parameter Description Stimulation Assay format 4-point dose U937 vs. IFN-γ concentration curve Assay throughput 48-well plate Activation cell line U937, ATCC CRL-1593.2 (lot no. 70036658) Number of TILs per well 1.5 × 10⁶ Number of U937 cells per well 4.0 × 10⁵, 2.0 × 10⁵, 1.0 × 10⁵, 0.5 × 10⁵ Co-culture media AIM-V (serum-free medium) containing IL-2 (300 IU/mL) Volume of media 1000 μL Number of replicate wells per 3 (all three wells are sourced from the same thawed vial of test article; an sample/per plate/per run average of 3 wells is used to determine IFNγ concentration). .. Period of stimulation 24 ± 0.5 hours at 37° C. in an incubator with 5% CO₂ Positive control Qualified TIL line Negative control Anti-HLA I&II antibodies Analysis Assay throughput 72-well plate Instrument ELLA automated ELISA System Software Simple Plex Runner and Simple Plex Explorer ELLA parameters Simple Plex 72-well IFN-γ 3rd generation Version 5 cartridge (cat. no. SPCKB-CS-002697), ProteinSimple, paired capture and detection antibodies conjugated to three (3) glass microtubes in series for triplicate repeated readout within each well.

Activation Assay:

Article Title: Methods and compositions for T-cell coculture potency assays and use with cell therapy products
Article Snippet: .. Step Parameter Description Stimulation Assay format 4-point dose U937 vs. IFN-γ concentration curve Assay throughput 48-well plate Activation cell line U937, ATCC CRL-1593.2 (lot no. 70036658) Number of TILs per well 1.5 × 10⁶ Number of U937 cells per well 4.0 × 10⁵, 2.0 × 10⁵, 1.0 × 10⁵, 0.5 × 10⁵ Co-culture media AIM-V (serum-free medium) containing IL-2 (300 IU/mL) Volume of media 1000 μL Number of replicate wells per 3 (all three wells are sourced from the same thawed vial of test article; an sample/per plate/per run average of 3 wells is used to determine IFNγ concentration). .. Period of stimulation 24 ± 0.5 hours at 37° C. in an incubator with 5% CO₂ Positive control Qualified TIL line Negative control Anti-HLA I&II antibodies Analysis Assay throughput 72-well plate Instrument ELLA automated ELISA System Software Simple Plex Runner and Simple Plex Explorer ELLA parameters Simple Plex 72-well IFN-γ 3rd generation Version 5 cartridge (cat. no. SPCKB-CS-002697), ProteinSimple, paired capture and detection antibodies conjugated to three (3) glass microtubes in series for triplicate repeated readout within each well.

other:

Article Title: Targeting host deoxycytidine kinase mitigates Staphylococcus aureus abscess formation
Article Snippet: Cell line ( Homo sapiens ) , U937 , ATCC , ATCC CRL-1593.2 , .

Article Title: LRRK2 maintains mitochondrial homeostasis and regulates innate immune responses to Mycobacterium tuberculosis
Article Snippet: Cell line ( Homo sapiens ) , U937 , ATCC , ATCC CRL-1593.2 , Cell line maintained in the Watson lab.

Article Title: Synthetic antibody mimetic compounds (SYAMS) targeting cancer, especially prostate cancer
Article Snippet: U937 Cells were purchased from ATCC (#CRL-1593.2), grown in Petri dishes as a suspension with RPMI-1640 medium supplemented with 10% HI-FBS and 1% penicillin-streptomycin.

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